T7 promoter lac o m c s l a c l p b r 3 2 2 o r i f 1 o r i. Please also check our more detailed, fully editable world map. Bgl 11 t 7 promoter lac operator xba 1 rbs ag atc tcg atc ccg cga aat taa tac gac tca cta tig ggg aat tgt gag cc,c, ata aca att ccc ctc tag aaa taa ttt. Included in the multiple cloning region are dual bstx i sites, which allow ef. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. As part of that effort, we supply carefully annotated files for common plasmids. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the vector map tb038. Note that the sequence is numbered by the pbr322 convention, so the t7 expression. T 7 promoter lac operator xba 1 rbs strep tag il taa tac gac tca cta tag ggg aat tgt gag cgg ata aca att ccc ctc tag aaa taa ttt tgt tta act tta aga agg aga tat acc atg gca. Terms and conditions 20042018 harvard medical school plasmid was created and is maintained by the dfhcc dna resource core at harvard medical schooldfhcc dna resource. T 7 promoter lac operator xba rbs ag atc tcg atc ccc, cc,a aat taa tac gac tca cta tig ggg aat tgt gag cc,g ata aca att ccc ctc tag aaa taa ttt. Use pet28b pdf on bb, construct the vector so that there are no nterminal additions and the dcoh protein will have a cterminal histidine tag. Promoter and cdna was excised and cloned into the noti site of part27. A repository of over 200,000 plasmids including protein structure initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms.
Please also check our more detailed, editable america. Novagen this second printing of the 10 th edition of the pet manual was published may, 2003. Social media communication internet network connection city skyscraper view world map. For publishing, design, printing, media, publications, arts, projects, presentations, for architects, designers and builders, business, logistics.
Unique sites except for the two bstx i sites are shown on the circle map. Pcr cloning vectors with 3 options for insert excision. The archive is devided into 7 main categories which consist of subcategories countries of the continent. Novagen is continually expanding and upgrading the pet system. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the circular map. Markus ralsers lab contains the insert triosephosphate isomerase 1 and is published in open biol. According to the brochure that i attached to this message pet28. Nina papavasilious lab contains the insert aid and is published in mol cell biol. It is used for t7 promoter driven expression of recombinant proteins with the addition of a 19 amino acid nterminal fusion tag containing a 6x histag followed by a thrombin protease cleavage site. Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.
Sequences inserted at the nde i site will be expressed in the. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on. Bacterial vector for expression of nterminally 6xhistagged proteins with a thrombin site. Figure 2 circular map and features for the pfbneo retroviral vector. A 0502 the pet44 vectors are designed for cloning and highlevel expression of peptide sequences fused with the 495 aa nustag protein. This is a free resource for the scientific community that is compiled by addgene.
Cloning sites are shown on the maps provided with your pet vector kit. Pdf world map from a different perspective, placing the american continent in the center. You can also submit other service inquiries here, including. Convenient expression of cloned genes in vitro or in vivo. Bacterial vector for expression of nterminally t7tagged proteins. Description the pet28alic vector was derived from expression plasmid pet28a novagen. Download vector layers and readytogo gis projects based on osm esri shape, geodatabase, geojson, pdf, csv, tab, pbf, xml, sql formats for qgis. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the. United states city plans vector street maps and full us maps in adobe illustrator, pdf and other vector formats. Us city maps vector urban plans in the adobe illustrator. Target sequences inserted between the nterminal signal sequence and cterminal cbd cex tag and histag sequences allow for potential export of the expressed protein. It therefore lacks the ribosome binding site and atg start codon present on the pet translation vectors.
C gaa aat acg act cac tat agg gga att gtg agc gga taa caa ttc ccc rcr aga aat aat rrr gtt taa ctt taa gaa gga gat t7 tag bamh1 ecor1 sacl sal 1 hind 111 not 1 xhol his tag. Two stop codons are included in the vector at the cterminal. Bglll t7 promoter lac operator xba 1 rbs aga tct cga tcc cgc gaa att aat acg act cac tat rgg gga att gtg agc gga taa caa ttc ccc tct aga aat aat ttt gtt taa ctt taa gaa gga gat 1 pelb leader bamh1 ecor1 sacl. Find msds or sds, a coa, data sheets and more information. This is a free resource for the scientific community that is compiled by addgene this page is informational only this vector is not available from addgene please contact the manufacturer for further details. The pet vectors 4 vector characteristics and cloning strategy 4 ligationindependent cloning lic of. World map with selected countries in black template. Free vector maps in ai, eps, pdf, svg, jpg and png formats. Bspd6i per 1 g wet biomass, and this yield was two orders of magnitude. An nde i or nco i site is available in many vectors for cloning into the aug start codon at the 5end of the insert coding sequence. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the circular. Download thousands of free vector maps, royalty free maps, world maps, us maps, map bundles in adobe illustrator, microsoft powerpoint, eps, pdf, png and jpg formats.
The complete nucleotide sequence and list of the complete nucleotide sequence and list of restriction sites can be found at. Use this option to ask our scientists to design your vector for free. The pht43 vector is a highlevel expression vector for recombinant protein production and secretion into the b. You are asked to create a vector that will express the protein called dcoh in bacteria information page from ncbi is attached in the pages handed out during class target.